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gmuG [2019-07-23 11:13:14]
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gmuG [2019-07-23 11:13:14]

beta-1,4-mannanase
Locus
BSU_05880
Isoelectric point
5.63
Molecular weight
40.68 kDa
Protein length
362 aa Sequence Blast
Gene length
1089 bp Sequence Blast
Function
glucomannan utilization
Product
beta-1,4-mannanase
Essential
no
E.C.
3.2.1.78
Synonyms
ydhT
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of glucomannan
  • Groups of genes → Secreted proteins

    • Gene

    Coordinates
    632,774 633,862

    The protein

    Catalyzed reaction/ biological activity

  • Random hydrolysis of (14)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans (according to Swiss-Prot)

    • Protein family

  • glycosyl hydrolase 26 family (single member, according to UniProt)

    • Structure

  • 2QHA, 3CBW mutant variant

    • Localization

  • extracellular (signal peptide) PubMed

    • Expression and Regulation

    Operons

    Genes
    gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • GmuR: repression, PubMed, in GmuR regulon
  • CcpA: repression, PubMed, in CcpA regulon
  • AbrB: activation, PubMed, in AbrB regulon

    • Regulation

  • induced by cellobiose (GmuR) PubMed
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    Biological materials

    Mutant

  • MGNA-C195 (ydhT::erm), available at the NBRP B. subtilis, Japan
  • BKE05880 (gmuG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAATGCAACTCCCCCATTC, downstream forward: _UP4_TGAATCCGTAATGTCACAAT
  • BKK05880 (gmuG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAATGCAACTCCCCCATTC, downstream forward: _UP4_TGAATCCGTAATGTCACAAT

    • References

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